How do you find the area of a electrode?

It depends on the nature of your electrode. Note that there are different equations for reversible, irreversible and quasi-reversible processes. Using the formula ECSA = Cdl /Cs electroactive area can be estimated.

How do you find the area under a curve in origin 8?

For Integration gadget, go to Gadgets:Integrateand click OK in the coming up dialog to bring up the yellow Region of Interest (ROI) box. Drag to position and resize the box to the area you want to calculate, then the Area and FWHM information will show up on the ROI top.

How do you find the area under a curve using Excel?

You can calculate its area easily with this formula: =(C3+C4)/2*(B4-B3). 2. Then you can drag the AutoFill handle of the formula cell down to calculate areas of other trapezoids. Note: The last trapezoid is between x=14 and x=15 under the curve.

How do you find the area under the peak?

The area of a peak is proportional to amount of the compound that is present. The area can be approximated by treating the peak as a triangle. The area of a triangle is calculated by multiplying the height of the peak times its width at half height.

What is the peak area in HPLC?

The area under a peak [peak area count] is a measure of the concentration of the compound it represents. This area value is integrated and calculated automatically by the computer data station. In this example, the peak for acrylamide in Sample A has 10 times the area of that for Sample B.

What is the unit of peak area in HPLC?

However it is measured, the units of peak area are the product of the x and y units. Thus, in a chromatogram where the x is time in minutes and y is volts, the area is in volts-minute. In absorption spectrum where the x is nm (nanometers) and y is absorbance, the area has the units of absorbance-nm.

How do you read a chromatogram?

The chromatogram is a graph that monitors the signal in the detector over time. As chemicals are detected by the instrument, the signal increases, and the chromatogram displays a “peak.” Each peak in the chromatogram indicates the presence of a chemical in the sample.

What is a DNA chromatogram?

A chromatogram (sometimes also called electropherogram) is the visual representation of a DNA sample produced by a sequencing machine (such as Applied Biosystems ABI PRISM 7700 Sequence Detection System). The green bars above chromatogram peaks high confidence scores.

How do you plot chromatogram?

Right click on the graph and select Create Label or select Chromatogram – Create Label on the main menu to add a line or text. An auxiliary graph where the chromatogram is always displayed in its original size. The graph is designated as an illustration of the cut-out performed in the main graph.

What is RF and how is it calculated?

In thin-layer chromatography, the retention factor (Rf) is used to compare and help identify compounds. The Rf value of a compound is equal to the distance traveled by the compound divided by the distance traveled by the solvent front (both measured from the origin).

Why is RF value calculated?

The amount that each component of a mixture travels can be quantified using retention factors (Rf). The retention factor of a particular material is the ratio of the distance the spot moved above the origin to the distance the solvent front moved above the origin.

Does higher RF mean more polar?

In general, the adsorptivity of compounds increases with increased polarity (i.e. the more polar the compound then the stronger it binds to the adsorbent). Non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value).

Can an RF be greater than 1?

Why or why not? -In order to have an Rfvalue greater than 1 the pigment would have to move further than the solvent. Since the pigment is carried by the solvent an Rfgreater than one is not possible.