Which stain is used for malaria parasite?

Giemsa stain
The Giemsa stain is used as the gold standard for the diagnosis of malaria on blood smears. The classical staining procedure requires between 30 and 45 min.

How do you stain malaria parasite using field stain?

Field stain B commercially available powder = 4.8 grams. Distilled water heated to 80 °C or keep at 60 °C for 30 min. = 600 mL. Mix well the field stain B until it is dissolved….

How do you stain malaria parasite?

Light microscopy of thick and thin stained blood smears remains the standard method for diagnosing malaria. It involves collection of a blood smear, its staining with Romanowsky stains and examination of the Red Blood Cells for intracellular malarial parasites.

Why is Giemsa staining commonly used in malaria surveys?

Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.

Why do we stain malaria parasite?

A properly stained blood film is critical for malaria diagnosis, especially for precise identification of malaria species. De-haemoglobinization of the thick film and staining take place at the same time. The ideal pH for demonstrating stippling of the parasites to allow proper species identification is 7.2.

How do you make a 10% Giemsa stain?

Make up a 10% Giemsa solution with distilled/deionized water buffered to pH 7.2. If only one slide is to be stained, you will require about 3 ml of prepared stain. Allow 3 drops of stock Giemsa solution (from the Pasteur pipette) to each millilitre of buffered water to give a 10% solution.

What is the purpose of field stain?

Field stain is a histological method for staining of blood smears. It is used for staining thick blood films in order to discover malarial parasites. Field’s stain is a version of a Romanowsky stain, used for rapid processing of the specimens.

How do you detect malaria parasite in thick film?

Thick and Thin Blood Smears for Malaria

1. A thick blood smear is a drop of blood on a glass slide. Thick blood smears are most useful for detecting the presence of parasites, because they examine a larger sample of blood.
2. A thin blood smear is a drop of blood that is spread across a large area of the slide.

Is Giemsa stain acidic or basic?

Principle of Giemsa Stain Azure and eosin are acidic dye that variably stains the basic components of the cells like the cytoplasm, granules etc. Methylene blue acts as the basic dye, which stains the acidic components, especially the nucleus of the cell.

Which mordant is used in Gram staining?

Gram’s Iodine
The mordant is Gram’s Iodine. This binds to the crystal violet making a large complex that adheres to the cell membrane. Gram’s Iodine is allowed to sit for 30 seconds then the decolorizor, 95% ethanol is added.

How do you prepare giemsa for staining?

Popular Answers (1)

1. Dissolve 3.8g of Giemsa powder into 250ml of methanol.
2. Heat the solution from step 1 to ~60oC.
3. Slowly add in 250ml of glycerin to the solution from step 2.
4. Filter the solution from step 3.
5. The solution needs to stand a period of time prior to use.

What are the steps for Gram staining?

The performance of the Gram Stain on any sample requires four basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram’s Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with …

How long does it take to stain a malaria blood film?

PROTOCOL FOR STAINING MALARIA BLOOD FILMS. If no parasites have been detected by the time the thick films are sufficiently dry for staining, further examination can be on these slides with a consequent increase in sensitivity. The thick films should be examined for around 10 to 15 minutes before being declared`negative’.

What kind of stain is used to diagnose malaria?

Laboratory diagnosis of malaria. Wright (Wright-Giemsa) stain. Used in hematology, this stain is not optimal for blood parasites. It can be used if rapid results are needed, but should be followed up when possible with a confirmatory Giemsa stain, so that Schüffner’s dots can be demonstrated.

How to recognize malaria parasites in blood films?

Examining blood films for malaria parasites By the end of this Unit you should be able to: • distinguish malaria parasites in thin blood films, and recognize and name the three stages of trophozoite, schizont and gametocyte

When to use Wright-Giemsa stain for malaria?

Laboratory diagnosis of malaria Wright (Wright-Giemsa stain Used in hematology, this stain is not optimal for blood parasites. It can be used if rapid results are needed, but should be followed up when possible with a confirmatory Giemsa stain, so that Schüffner’s dots can be demonstrated.