What is BspHI?
Thermo Scientific PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (isoschizomers: BspHI, CciI, RcaI). Note: Also available as a FastDigest enzyme for rapid DNA digestion.
How do you find restriction enzyme sites?
The option Find Restriction Sites… from the “Tools”→“Cloning” menu or the context menu allows you to find and annotate restriction sites on a nucleotide sequence.
What is the recognition sequence for PagI?
| Enzyme | Source | Recognition sequence |
|---|---|---|
| PagI | Pseudomonas alcaligenes Sau 14-027 | 5′ TCATGA 3′ AGTACT |
| PalI | Providencia alcalifaciens | 5′ GGCC 3′ CCGG |
| PamI | Phormidium ambiguum | 5′ TGCGCA 3′ ACGCGT |
| PamII | Phormidium ambiguum | 5′ GRCGYC 3′ CYGCRG |
How to analyze a restriction enzyme cutting map?
Restriction Enzyme Map Analysis GenScript restriction enzyme map analysis tools help you analyze restriction enzyme cutting maps. GenScript Home English Chinese Japanese Sign In Please Sign In
What are the sites for restriction enzymes in DNA?
Maps sites for restriction enzymes, a.k.a. restriction endonucleases, in DNA sequences. Also does virtual digestion. What`s New? No non-base letters.
How to find a restriction enzyme in Neb?
Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. Enzyme Finder | NEB Enzyme Finder version {{appVersion}}
Which is Thermo Fisher Scientific pagi restriction enzyme?
Order requests are processed and shipped by your local distributor. Learn more Thermo Scientific PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (isoschizomers: BspHI, CciI, RcaI).